Authors: M. Balu, K. M. Kelly, C. B. Zachary, R. M. Harris, T. B. Krasieva, K. König, A. J. DurkinRead more
3D MPM imaging of healthy human skin in vivo based on TPEF (green) of NADH/FAD/elastin, and SHG (blue) of collagen. (Left) Cross-sectional MPM image (XZ scan). This image shows the stratum corneum (SC), stratum spinosum (SS, composed of mature keratinocytes), stratum basale (SB, formed by proliferating and non-proliferating keratinocytes), stratum papillare (SP, exhibits papillary collagen), blood vessels (BV), and stratum reticulare (SR, contains dermal collagen and elastin). Scale bar is 20 μm. (Right) En-face MPM images (XY scans) of skin at different depths corresponding to the cross-sectional image on the left.